Chinese Journal of Modern Operative Surgery  2017, Vol. 21 Issue (2): 85-89 DOI: 10．16260/j．cnki．1009 －2188．2017．02．002 |
| Original Research |
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| The Expression of Tumor Associated Macrophages and Cytokines in the Triple-negative Breast Cancer Patients after the Surgery |
| WANG Jian-guo，LI Shao-jie
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| Department of General Surgery，The Central Hospital of Xiangtan，Xiangtan 411100，Hunan，China |
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Abstract ObjectiveTo explore the expressional value of tumor associated macrophages(TAMs)and cytokines in the prognosis of triple-negative breast cancer(TNBC)．MethodsImmunohistochemistry(IHC)and S-P staining method were used to detect the TAMs marker CD68 expression in 48 TNBC samples．Expressions of key cytokines including interleukin-6(IL-6)，IL-10，IL-12，IL-1β，chemokine(C-C motif)ligand-5(CCL-5)and macrophage inflammatory protein-2(MIP-2)were quantified by RT-PCR and enzyme-linked immunosorbent assay(ELISA)．The correlation between CD68 expression and prognosis was analyzed．Results34 cases(70．8%)out of 48 TNBC samples appeared CD68-positive expression．Compared to low infiltrated samples，IL-6 and CCL-5 were up-regulated in the high infiltrated tumors samples(P ＜0．01)，but there was no statistical difference in other cytokines between low and high infiltrated tumor samples．The survival rate of high infiltrated tumor samples were lower than that of low infiltrated tumor sample(P ＜0．05)．ConclusionsThe expression of TAMs were up-regulated in most of TNBC patients after the surgery．Its expression suggested unfavorable prognosis especially in high-infiltrated group．Those tumors with more macrophage also had elevated expression of cytokine(IL-6)and chemotactic factor(CCL-5)，both of which have potency to be clinical index and drug target for TNBC．
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Received: 29 December 2016
Published: 25 May 2018
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Corresponding Authors:
WANG Jian-guo
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| About author:: 王建国，男，40岁，湖南省湘潭市中心医院普外二(乳甲)科主任，副主任医师。 |
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